Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants.

Efficient, antibiotic marker-free transformation of a dicot and a monocot crop with glutamate 1-semialdehyde aminotransferase selectable marker genes

GIANCASPRO, ANGELICA;GADALETA, Agata;
2016-01-01

Abstract

Antibiotic-free, efficient in vitro selection in plant genetic engineering can improve risk perception and speed up pre-market scrutiny of genetically modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentially be applied, with minor adaptations, to many other monocot and dicot crop plants.
2016
978-1-4939-3288-7
978-1-4939-3289-4
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/173590
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