Various Lactobacillus reuteri strains were investigated as whole cell catalysts for the bioreduction of acetophenone into optically active (R)-1-phenylethanol. L. reuteri DSM 20016 strain gave an almost complete substrate conversion, in a short reaction time and enantiomeric excess up to 99%. The acetophenone bioreduction was used as a model reaction to optimize temperature and reducing equivalent source (glucose, lactose, cheese way and lignocellulosic hydrolysates) to accomplish the biotransformation. The reduction of acetophenones into optically active (R)-1-arylethanols was also exploited to study L. reuteri DSM 20016 substrate specificity. In most of the cases, optically active (R)-1-arylethanols have been obtained with both excellent chemical and optical yields and with (R)-enantiopreference, through a cheap, simple and efficient process.

Cheap and environmentally sustainable stereoselective arylketones reduction by Lactobacillus reuteri whole cells

PERNA, FILIPPO;RICCI, Maria Antonietta;SCILIMATI, Antonio;MENA, MARIA CONCETTA;PISANO, ISABELLA;PALMIERI, Luigi;AGRIMI, GENNARO
;
VITALE, PAOLA
2016-01-01

Abstract

Various Lactobacillus reuteri strains were investigated as whole cell catalysts for the bioreduction of acetophenone into optically active (R)-1-phenylethanol. L. reuteri DSM 20016 strain gave an almost complete substrate conversion, in a short reaction time and enantiomeric excess up to 99%. The acetophenone bioreduction was used as a model reaction to optimize temperature and reducing equivalent source (glucose, lactose, cheese way and lignocellulosic hydrolysates) to accomplish the biotransformation. The reduction of acetophenones into optically active (R)-1-arylethanols was also exploited to study L. reuteri DSM 20016 substrate specificity. In most of the cases, optically active (R)-1-arylethanols have been obtained with both excellent chemical and optical yields and with (R)-enantiopreference, through a cheap, simple and efficient process.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/145275
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