The effects of lycopene-enriched extenders on in vitro quality characteristics and lipid peroxidation of turkey semen after both chilled and frozen storage were evaluated. Five pools of semen diluted in extenders containing 0, 0.05 or 0.1 mg/mL lycopene were stored either at 5°C for 48 h or cryopreserved as pellets. Mobility, viability, osmotic resistance, DNA integrity as well as lipid peroxidation (malonaldehyde production) of spermatozoa were evaluated in fresh, chilled and frozen sperm. Semen quality was generally reduced after storage, especially post-freezing. However, in semen extended with the highest dose of lycopene, neither viability and osmotic-resistance of chilled sperm, nor DNA integrity of frozen sperm, differed significantly from those of fresh semen. Lipid peroxidation was higher in refrigerated than in fresh or cryopreserved spermatozoa. Anyway, spermatozoa chilled in lycopene-enriched extenders had significant lower malonaldehyde levels than those chilled without lycopene, whereas in frozen semen the addition of lycopene contributed to maintain the lipid peroxidation no different from that scored in fresh semen. In conclusion, lycopene improved the survival of turkey spermatozoa after liquid-storage and protected their DNA integrity against cryodamage. The positive effect of lycopene addition to extenders was likely due to its role in limiting the amount of sperm lipid peroxidation after both refrigeration and cryopreservation.
Effects of lycopene on in vitro quality and lipid peroxidation in refrigerated and cryopreserved turkey spermatozoa
CENTODUCATI, GERARDO;SANTACROCE, MARIA;
2012-01-01
Abstract
The effects of lycopene-enriched extenders on in vitro quality characteristics and lipid peroxidation of turkey semen after both chilled and frozen storage were evaluated. Five pools of semen diluted in extenders containing 0, 0.05 or 0.1 mg/mL lycopene were stored either at 5°C for 48 h or cryopreserved as pellets. Mobility, viability, osmotic resistance, DNA integrity as well as lipid peroxidation (malonaldehyde production) of spermatozoa were evaluated in fresh, chilled and frozen sperm. Semen quality was generally reduced after storage, especially post-freezing. However, in semen extended with the highest dose of lycopene, neither viability and osmotic-resistance of chilled sperm, nor DNA integrity of frozen sperm, differed significantly from those of fresh semen. Lipid peroxidation was higher in refrigerated than in fresh or cryopreserved spermatozoa. Anyway, spermatozoa chilled in lycopene-enriched extenders had significant lower malonaldehyde levels than those chilled without lycopene, whereas in frozen semen the addition of lycopene contributed to maintain the lipid peroxidation no different from that scored in fresh semen. In conclusion, lycopene improved the survival of turkey spermatozoa after liquid-storage and protected their DNA integrity against cryodamage. The positive effect of lycopene addition to extenders was likely due to its role in limiting the amount of sperm lipid peroxidation after both refrigeration and cryopreservation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
