Electrical parameters of extensor digitorum longus (EDL) muscles and their contralaterals were measured ''in vitro'' at 30 degrees C by a computerized two intracellular microelectrode technique after ischaemia and postischaemic reflow. In some muscles the adenosine triphosphate (ATP) levels are also measured. Ischaemia led to a 39% reduction of Cl- conductance (G(Cl)), whereas reperfusion increase G(Cl) by 18% with respect to contralateral control muscles. Ischaemia and reperfusion increased K+ conductance (G(K)) by 21% and 68%, respect ively; this increased was reversed by 50 mu M glybenclamide, suggesting an involvement of ATP-sensitive K+ channels. A statistically significant hyperpolarization and increase in excitability was observed after ischaemia, whereas after the reflow period the fibres were depolarized and less excitable. Ischaemia and reperfusion lowered the intracellular ATP content by 18% and 64%, respectively.

Effects of ischemia and postischemic reflow on the passive and active electrical parameters of rat skeletal muscle fibers

TRICARICO, Domenico;CONTE, Diana
1994-01-01

Abstract

Electrical parameters of extensor digitorum longus (EDL) muscles and their contralaterals were measured ''in vitro'' at 30 degrees C by a computerized two intracellular microelectrode technique after ischaemia and postischaemic reflow. In some muscles the adenosine triphosphate (ATP) levels are also measured. Ischaemia led to a 39% reduction of Cl- conductance (G(Cl)), whereas reperfusion increase G(Cl) by 18% with respect to contralateral control muscles. Ischaemia and reperfusion increased K+ conductance (G(K)) by 21% and 68%, respect ively; this increased was reversed by 50 mu M glybenclamide, suggesting an involvement of ATP-sensitive K+ channels. A statistically significant hyperpolarization and increase in excitability was observed after ischaemia, whereas after the reflow period the fibres were depolarized and less excitable. Ischaemia and reperfusion lowered the intracellular ATP content by 18% and 64%, respectively.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/135827
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