A fast, simple, and sensitive HPLC–FD method is described for determination of ochratoxin A (OTA) in pig kidney and muscle; a small mass (<2.5 g) of sample and a relatively small volume (<15 mL) of a non-halogenated extraction solvent are required. Ochratoxin B, systematically absent from all the samples investigated, was used as internal standard. Liquid–liquid partition was used for sample clean-up. Recoveries at the 1 ng g–1 level were 86±15% and 74±8% for kidney and muscle, respectively, and detection limits were 0.14 and 0.15 ng g–1. Clean-up by solid-phase extraction (SPE) is required for pig liver. A survey of the OTA content of tissues of pigs slaughtered in southern Italy revealed that 52 out of 54 analysed samples were contaminated; the OTA concentration in kidney ranged between 0.26 and 3.05 ng g–1. The effect of measurement precision on compliance with legal limits is also discussed.
Determination of ochratoxin A in pig tissue by liquid-liquid extraction/partition and high performance liquid chromatography
TANTILLO, Giuseppina;PALMISANO, Francesco
2004-01-01
Abstract
A fast, simple, and sensitive HPLC–FD method is described for determination of ochratoxin A (OTA) in pig kidney and muscle; a small mass (<2.5 g) of sample and a relatively small volume (<15 mL) of a non-halogenated extraction solvent are required. Ochratoxin B, systematically absent from all the samples investigated, was used as internal standard. Liquid–liquid partition was used for sample clean-up. Recoveries at the 1 ng g–1 level were 86±15% and 74±8% for kidney and muscle, respectively, and detection limits were 0.14 and 0.15 ng g–1. Clean-up by solid-phase extraction (SPE) is required for pig liver. A survey of the OTA content of tissues of pigs slaughtered in southern Italy revealed that 52 out of 54 analysed samples were contaminated; the OTA concentration in kidney ranged between 0.26 and 3.05 ng g–1. The effect of measurement precision on compliance with legal limits is also discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
