In mammals, T cells develop along two discrete pathways characterized by expression of either the alpha beta or the gamma delta T cell receptors. Human, mouse, and dog display a low peripheral blood gamma delta T cell percentage, while sheep accounts for a high proportion of gamma delta T lymphocytes. In all these species, the genomic organization of the T cell receptor gamma (TRG) locus is well known. To gain further insight into the evolutionary significance of the gamma delta T cell lineage, the present study has defined the genomic organization of the TRG locus in rabbit (Oryctolagus cuniculus), another mammalian gamma delta high species, as deduced from the genome assembly. The rabbit TRG locus spans about 70 kb and consists of ten TRGV, two TRGJ genes, and one TRGC gene located 5' to 3' in the locus. When we compared the rabbit sequence with the human, mouse, sheep, and dog counterparts, a higher identity with human as well as sheep with respect to mouse and dog was evident, providing that in the different mammalian species, the TRG locus appears to have evolved independently without any correlation with the gamma delta condition. The complete sequence of the rabbit TRG locus described here provides also a resource for supporting functional studies especially in the context of the gamma delta T cell function.

Structural and comparative analysis of the T cell receptor gamma (TRG) locus in Oryctolagus cuniculus

CICCARESE, Salvatrice Maria;ANTONACCI, Rachele
2012

Abstract

In mammals, T cells develop along two discrete pathways characterized by expression of either the alpha beta or the gamma delta T cell receptors. Human, mouse, and dog display a low peripheral blood gamma delta T cell percentage, while sheep accounts for a high proportion of gamma delta T lymphocytes. In all these species, the genomic organization of the T cell receptor gamma (TRG) locus is well known. To gain further insight into the evolutionary significance of the gamma delta T cell lineage, the present study has defined the genomic organization of the TRG locus in rabbit (Oryctolagus cuniculus), another mammalian gamma delta high species, as deduced from the genome assembly. The rabbit TRG locus spans about 70 kb and consists of ten TRGV, two TRGJ genes, and one TRGC gene located 5' to 3' in the locus. When we compared the rabbit sequence with the human, mouse, sheep, and dog counterparts, a higher identity with human as well as sheep with respect to mouse and dog was evident, providing that in the different mammalian species, the TRG locus appears to have evolved independently without any correlation with the gamma delta condition. The complete sequence of the rabbit TRG locus described here provides also a resource for supporting functional studies especially in the context of the gamma delta T cell function.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/132318
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