Purpose: To evaluate circulating CD4+CD25+ regulatory T-cell populations in patients with autoimmune uveitis and to assess whether T-regulatory cell populations correlate with clinical features. Methods: Sixty-four patients with noninfectious uveitis were enrolled. Following isolation and purification of peripheral blood mononuclear cells, Tregs were analyzed by assessing FOXP3 expression on T cells with an APC anti-human FOXP3 staining kit. Samples were simultaneously stained with anti-CD25-PeCy7, anti-CD3- FITC, anti-CD4-PercP, and anti-CD152 (CTLA-4)-PE monoclonal antibodies. Results: A decrease in CD4+CD25+FOXP3+ T cells was detected in patients with active uveitis compared with healthy controls (p < .05). In patients achieving clinical remission, the percentage of CD4+CD25+FOXP3+ T cells increased up to values comparable to those of healthy controls, in step with an increased expression of CTLA4. Conclusion: Therapy of autoimmune uveitis results in the increase of T-regulatory lymphocyte population and restoration of their functional state. These changes are likely to contribute to the patients' clinical improvement or disease regression.
Treg lymphocytes in autoimmune uveitis
DAMMACCO, ROSANNA;GUERRIERO, Silvana
2012
Abstract
Purpose: To evaluate circulating CD4+CD25+ regulatory T-cell populations in patients with autoimmune uveitis and to assess whether T-regulatory cell populations correlate with clinical features. Methods: Sixty-four patients with noninfectious uveitis were enrolled. Following isolation and purification of peripheral blood mononuclear cells, Tregs were analyzed by assessing FOXP3 expression on T cells with an APC anti-human FOXP3 staining kit. Samples were simultaneously stained with anti-CD25-PeCy7, anti-CD3- FITC, anti-CD4-PercP, and anti-CD152 (CTLA-4)-PE monoclonal antibodies. Results: A decrease in CD4+CD25+FOXP3+ T cells was detected in patients with active uveitis compared with healthy controls (p < .05). In patients achieving clinical remission, the percentage of CD4+CD25+FOXP3+ T cells increased up to values comparable to those of healthy controls, in step with an increased expression of CTLA4. Conclusion: Therapy of autoimmune uveitis results in the increase of T-regulatory lymphocyte population and restoration of their functional state. These changes are likely to contribute to the patients' clinical improvement or disease regression.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
