There are conflicting data on the biological and prognostic significance of disseminated tumour cells (DTCs) in the bone marrow of colorectal cancer patients since bone metastasis are rare in this disease. The study aimed to determine the origin of bone marrow -DTCs using human colorectal cancer cells in in vivo and in vitro experimental settings. METHOD: CD1 nude female mice were xenotransplanted with SW620 cells (colorectal cancer cell line isolated from a male patient) injected in the colon wall. At autopsy, the presence of SW620 in the bone marrow (BM), colon and other organs/tissues was recognized by detection of the epithelial marker cytokeratin-19 (CK19) and Y-chromosome. In addition SW620 cells or their conditioned media were cultured with human BM cells. RESULTS: Macroscopically evident CK19+/Y-chromosome+ tumours developed only in five mice receiving SW620 cells while putative DTCs (CK19+) were found in the bone marrow of all treated mice. Most of these CK19+ cells were Y-chromosome-negative, only few being Y-chromosome-positive. In vitro SW620 cells or their conditioned medium induced CK19 expression in cultured human bone marrow cells. CONCLUSION: Experimental colorectal cancer can induce the appearance of two distinct CK19+ cell populations in the bone marrow, one of metastatic origin and the other of murine origin. These findings suggest that bone marrow cells may undergo phenotypic modifications induced by cancer cells.

Disseminated tumour cells in bone marrow in experimental colon cancer: metastatic or resident?

Barone, M.;Altomare, D. F.;ROTELLI, MARIA TERESA;Scavo, M. P.;Piscitelli, D.;DE TULLIO, NICOLA;BOCALE, DOMENICA;Di Leo, A.
2013

Abstract

There are conflicting data on the biological and prognostic significance of disseminated tumour cells (DTCs) in the bone marrow of colorectal cancer patients since bone metastasis are rare in this disease. The study aimed to determine the origin of bone marrow -DTCs using human colorectal cancer cells in in vivo and in vitro experimental settings. METHOD: CD1 nude female mice were xenotransplanted with SW620 cells (colorectal cancer cell line isolated from a male patient) injected in the colon wall. At autopsy, the presence of SW620 in the bone marrow (BM), colon and other organs/tissues was recognized by detection of the epithelial marker cytokeratin-19 (CK19) and Y-chromosome. In addition SW620 cells or their conditioned media were cultured with human BM cells. RESULTS: Macroscopically evident CK19+/Y-chromosome+ tumours developed only in five mice receiving SW620 cells while putative DTCs (CK19+) were found in the bone marrow of all treated mice. Most of these CK19+ cells were Y-chromosome-negative, only few being Y-chromosome-positive. In vitro SW620 cells or their conditioned medium induced CK19 expression in cultured human bone marrow cells. CONCLUSION: Experimental colorectal cancer can induce the appearance of two distinct CK19+ cell populations in the bone marrow, one of metastatic origin and the other of murine origin. These findings suggest that bone marrow cells may undergo phenotypic modifications induced by cancer cells.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/131141
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