A survey on 54 paired kidneys and muscle samples from pigs slaughtered in Southern Italy, has been carried out in order to check the presence of ochratoxin A. Both competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) and high-performance liquid chromatography with fluorescence detection (HPLC-FD) were used to determine ochratoxin levels. Mean and median value for OTA in kidneys were 0.29 and 0.25 ng/g, respectively. Mean and median values found for muscle (0.024 and 0.01 ng/g) are significantly lower than those reported in other studies demonstrating that, irrespective of the geographical provenance of pigs, OTA incidence is far from representing a real concern for consumers. Results obtained plotting ELISA vs. HPLC results show that ELISA tends to slightly underestimate the OTA content compared to HPLC; nevertheless, ELISA remains an invaluable tool as rapid screening semiquantitative technique.

Ochratoxin A determination in paired kidneys and muscle samples from swines slaughtered in southern Italy

PALMISANO, Francesco;TANTILLO, Giuseppina
2006-01-01

Abstract

A survey on 54 paired kidneys and muscle samples from pigs slaughtered in Southern Italy, has been carried out in order to check the presence of ochratoxin A. Both competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) and high-performance liquid chromatography with fluorescence detection (HPLC-FD) were used to determine ochratoxin levels. Mean and median value for OTA in kidneys were 0.29 and 0.25 ng/g, respectively. Mean and median values found for muscle (0.024 and 0.01 ng/g) are significantly lower than those reported in other studies demonstrating that, irrespective of the geographical provenance of pigs, OTA incidence is far from representing a real concern for consumers. Results obtained plotting ELISA vs. HPLC results show that ELISA tends to slightly underestimate the OTA content compared to HPLC; nevertheless, ELISA remains an invaluable tool as rapid screening semiquantitative technique.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/129855
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 83
  • ???jsp.display-item.citation.isi??? 75
social impact