In this study we evaluated the in vitro variability of flaA in Campylobacter coli strains (Co19 and Co29) isolated from distinct poultry farms. Strains were characterized by flaA PCR-RFLP and subcultured in absence or presence of Salmonella enterica (S. Gallinarum) as a host competitor in poultry. Only transition mutations were detected and no flaA-flaB recombination events were observed. The highest ratio of flaA mutations was detected in the presence of competitor (1x10-6 and 4.8x10-6 for Co19 and Co29, respectively). While, transitions in absence of competitor were observed only in the strain Co29 (5.8x10-7). Data obtained in this investigation represent a preliminary study aimed to evaluate the possible linkage between specific haplotypes and their proneness to acquire point mutations which in turn might help strains to gain a better fitness for survival within and outside hosts.

Variability of Point Mutation Rates in Campylobacter coli: Preliminary Data and Observations.

CIRCELLA, ELENA;PAZZANI, Carlo;CAMARDA, Antonio;Pugliese N.
2014-01-01

Abstract

In this study we evaluated the in vitro variability of flaA in Campylobacter coli strains (Co19 and Co29) isolated from distinct poultry farms. Strains were characterized by flaA PCR-RFLP and subcultured in absence or presence of Salmonella enterica (S. Gallinarum) as a host competitor in poultry. Only transition mutations were detected and no flaA-flaB recombination events were observed. The highest ratio of flaA mutations was detected in the presence of competitor (1x10-6 and 4.8x10-6 for Co19 and Co29, respectively). While, transitions in absence of competitor were observed only in the strain Co29 (5.8x10-7). Data obtained in this investigation represent a preliminary study aimed to evaluate the possible linkage between specific haplotypes and their proneness to acquire point mutations which in turn might help strains to gain a better fitness for survival within and outside hosts.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/128313
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