Three adult goats, seronegative to caprine herpesvirus 1 (CpHV.1), were intravaginally inoculated with BA.1 strain of CpHV.1. The animals were kept under observation for 1 month and daily both clinical examinations and white blood cell count were performed. Ocular, nasal, rectal and vaginal swabs and heparinized blood samples were collected every day to attempt virus isolation on cell cultures and detect viral DNA by polymerase chain reaction (PCR) assay. The virus was isolated and detected by PCR only from the vaginal swabs for 5-7 days post-infection. The animals showed transient fever and leukopenia and typical necrotic lesions on the vulva and vagina.
Experimental intravaginal infection of goats with caprine herpesvirus
TEMPESTA, Maria;PRATELLI, Annamaria;CAMERO M.;BUONAVOGLIA, Domenico;GRECO, Grazia;BUONAVOGLIA, Canio
2000-01-01
Abstract
Three adult goats, seronegative to caprine herpesvirus 1 (CpHV.1), were intravaginally inoculated with BA.1 strain of CpHV.1. The animals were kept under observation for 1 month and daily both clinical examinations and white blood cell count were performed. Ocular, nasal, rectal and vaginal swabs and heparinized blood samples were collected every day to attempt virus isolation on cell cultures and detect viral DNA by polymerase chain reaction (PCR) assay. The virus was isolated and detected by PCR only from the vaginal swabs for 5-7 days post-infection. The animals showed transient fever and leukopenia and typical necrotic lesions on the vulva and vagina.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
