A texture-based image processing approach for the description of human oocyte cytoplasm

The purpose of this paper is to develop a diagnostic tool that can analyze light microscope images of human oocytes and derive a description of the oocyte cytoplasm that is useful for quality assessment in assisted insemination. The proposed approach includes three main phases: 1) segmentation; 2) feature extraction; and 3) clustering. In the segmentation phase, a region of interest inside the cytoplasm is extracted through morphological operators and the Hough transform. In the second phase, regions that result from segmentation are processed through a multiresolution texture analysis to extract a set of features that describe different levels of cytoplasm granularity. To this aim, we evaluate some statistics in the Haar wavelet transform domain. Finally, the extracted features are used to cluster oocytes according to different levels of granularity. This approach is made by fuzzy clustering. Experimental results on a collection of microscope images of oocytes are reported to show the effectiveness of the proposed approach. In addition, comparison with alternative methods for feature extraction and clustering is performed.