Bladder cancer (BC) is a major cause of mortality worldwide as it currently lacks fully reliable markers of disease outcome and effective molecular targets for therapy. A shift to glycolysis and an upregulation of glucose transport (GLUT1) were reported in BC [1]. Therefore, to verify whether the increase in glycolysis in BC tissues may be associated to mitochondrial dysfunction, the activity of respiratory complexes and the expression level of some subunits of these complexes and of lactate dehydrogenase (LDH) were measured in BC tissues and in non-tumor adjacent tissues (controls). In the same samples, the mitochondrial DNA to nuclear DNA ratio and the expression level of proteins involved in mitochondrial biogenesis (PGC-1, NRF1 and TFAM), mitochondrial dynamics (MFN2 and Drp1) and mitochondrial protein degradation (CLPP) were also measured. Altered expression levels of these proteins were found in BC tissues compared to control tissues suggesting that mitochondrial alterations may be present in this cancer. References 1) Benjamin L. et al., 2018 Metabolic changes in bladder cancer. Urologic Oncology:36: 327–337

Mitochondrial alterations in bladder cancer

Anna Signorile;
2019

Abstract

Bladder cancer (BC) is a major cause of mortality worldwide as it currently lacks fully reliable markers of disease outcome and effective molecular targets for therapy. A shift to glycolysis and an upregulation of glucose transport (GLUT1) were reported in BC [1]. Therefore, to verify whether the increase in glycolysis in BC tissues may be associated to mitochondrial dysfunction, the activity of respiratory complexes and the expression level of some subunits of these complexes and of lactate dehydrogenase (LDH) were measured in BC tissues and in non-tumor adjacent tissues (controls). In the same samples, the mitochondrial DNA to nuclear DNA ratio and the expression level of proteins involved in mitochondrial biogenesis (PGC-1, NRF1 and TFAM), mitochondrial dynamics (MFN2 and Drp1) and mitochondrial protein degradation (CLPP) were also measured. Altered expression levels of these proteins were found in BC tissues compared to control tissues suggesting that mitochondrial alterations may be present in this cancer. References 1) Benjamin L. et al., 2018 Metabolic changes in bladder cancer. Urologic Oncology:36: 327–337
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11586/243122
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