The anodic behavior of pseudouridine, a modified nucleoside proposed as tumor marker, was studied at a mercury electrode. A method for its detn. in urine samples by HPLC with indirect anodic polarog. detection was developed. The method is simple and highly selective and permits the direct injection of urine after diln. (1:20) with mobile phase. Creatinine, which also gave an anodic response on mercury, could be simultaneously detd. and used as an internal std. for pseudouridine. This allowed detn. of the pseudouridine/creatinine ratio in a single run on the same specimen and the use of urine samples collected randomly instead of a 24-h collection. The within-day coeff. of variation for pseudouridine/creatinine peak area ratios was 3.4%.
Simultaneous determination of pseudouridine and creatinine in urine by HPLC with polarographic detection.
PALMISANO, Francesco;
1989-01-01
Abstract
The anodic behavior of pseudouridine, a modified nucleoside proposed as tumor marker, was studied at a mercury electrode. A method for its detn. in urine samples by HPLC with indirect anodic polarog. detection was developed. The method is simple and highly selective and permits the direct injection of urine after diln. (1:20) with mobile phase. Creatinine, which also gave an anodic response on mercury, could be simultaneously detd. and used as an internal std. for pseudouridine. This allowed detn. of the pseudouridine/creatinine ratio in a single run on the same specimen and the use of urine samples collected randomly instead of a 24-h collection. The within-day coeff. of variation for pseudouridine/creatinine peak area ratios was 3.4%.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
