Herein, this work reports the first example of second-generation wearablebiosensor arrays based on a printed electrode technology involving awater-based graphite ink, for the simultaneous detection of l-lactate andd-glucose. The water-based graphite ink is deposited onto a flexiblepolyethylene terephthalate sheet, namely stencil-printed graphite (SPG)electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10]asanosmiumredox polymer to shuttle the electrons from the redox center of lactate oxidasefromAerococcus viridans(LOx) and gluocose oxidase fromAspergillus niger(GOx). The proposed biosensor array exhibits a limit of detection as low as(9.0±1.0)×10−6m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10]and(3.0±0.5)×10−6m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32𝝁Amm−1for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4𝝁Amm−1forGOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when testedin buffer and artificial sweat and is endowed with an operational/storagestability of≈80% of the initial signal retained after 20 days. Finally, theproposed array is integrated in a wristband and successfully tested for thecontinuous monitoring of l-lactate and d-glucose in a healthy volunteerduring daily activity. This is foreseen as a real-time wearable device forsport-medicine and healthcare applications

Water‐Based Conductive Ink Formulations for Enzyme‐Based Wearable Biosensors

Tricase, Angelo;Valentino, Marlene;Ditaranto, Nicoletta
Investigation
;
Macchia, Eleonora;Franco, Cinzia Di;Piscitelli, Matteo;Scamarcio, Gaetano;Torsi, Luisa
;
Bollella, Paolo
2023-01-01

Abstract

Herein, this work reports the first example of second-generation wearablebiosensor arrays based on a printed electrode technology involving awater-based graphite ink, for the simultaneous detection of l-lactate andd-glucose. The water-based graphite ink is deposited onto a flexiblepolyethylene terephthalate sheet, namely stencil-printed graphite (SPG)electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10]asanosmiumredox polymer to shuttle the electrons from the redox center of lactate oxidasefromAerococcus viridans(LOx) and gluocose oxidase fromAspergillus niger(GOx). The proposed biosensor array exhibits a limit of detection as low as(9.0±1.0)×10−6m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10]and(3.0±0.5)×10−6m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32𝝁Amm−1for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4𝝁Amm−1forGOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when testedin buffer and artificial sweat and is endowed with an operational/storagestability of≈80% of the initial signal retained after 20 days. Finally, theproposed array is integrated in a wristband and successfully tested for thecontinuous monitoring of l-lactate and d-glucose in a healthy volunteerduring daily activity. This is foreseen as a real-time wearable device forsport-medicine and healthcare applications
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11586/444400
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