The possibility of identifying or tracing the primary product, mainly the breed of origin, by the use of biologic markers, is an important goal for National goat cheese. In this field, great interest is paid to the genetic polymorphism of milk protein. In order to perform an investigation on the possibility of tracing the source milk in goat dairy products, a study was carried out on the use of molecular techniques for the analysis of goat cheese. Fourteen cheeses with different origins were analysed. A good quality DNA, obtained by the use of a commercial kit, was successfully amplified and submitted to different molecular techniques for the analysis of the genetic polymorphism at alpha sub(s1)-casein, alpha sub(s2)-casein and kappa-casein loci. In particular, kappa-casein locus allowed the differentiation between goat breeds showing alleles with different distribution, as well as the discrimination between the caprine, ovine, and bovine species evaluated on pools of individual DNA extracted from milk. The analysis will aim both to detect frauds and to identify particular alleles characterising local breeds which are interesting for the safeguard of both local livestock and typical dairy products
A molecular approach for the traceability of goat dairy products
PIERAGOSTINI, Elisa
2004-01-01
Abstract
The possibility of identifying or tracing the primary product, mainly the breed of origin, by the use of biologic markers, is an important goal for National goat cheese. In this field, great interest is paid to the genetic polymorphism of milk protein. In order to perform an investigation on the possibility of tracing the source milk in goat dairy products, a study was carried out on the use of molecular techniques for the analysis of goat cheese. Fourteen cheeses with different origins were analysed. A good quality DNA, obtained by the use of a commercial kit, was successfully amplified and submitted to different molecular techniques for the analysis of the genetic polymorphism at alpha sub(s1)-casein, alpha sub(s2)-casein and kappa-casein loci. In particular, kappa-casein locus allowed the differentiation between goat breeds showing alleles with different distribution, as well as the discrimination between the caprine, ovine, and bovine species evaluated on pools of individual DNA extracted from milk. The analysis will aim both to detect frauds and to identify particular alleles characterising local breeds which are interesting for the safeguard of both local livestock and typical dairy productsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
