A revegetation process of a closed urban solid waste landfill was initiated in 2009 for the environmental restoration of the site. The landfill is located in Foggia district in the North part of the Apulia region. Growth experiments in pots and in open field were carried out on eight plant species in order to select those that could be more appropriate in the particular extreme field conditions (climate, high slope of the walls, bad leachate control). In particular, plant growth was assessed in two different soil types (agricultural and leachate contaminated soil). Among the different species assessed, Pistacia lentiscus L. and Puccinellia borreri. L. were selected because of their performance in the contaminated soil and mostly since they are typical native Mediterranean plant species. Rhizosphere and bulk soil of these two plant species, sampled from the pots, has been characterized by biochemical and molecular methods in order to highlight shift in the composition of the microbial community caused by the presence of leachate. Enzymatic activities (dehydrogenase, fluorescein diacetate hydrolysis, ?-glucosidase and protease) and microbial biomass C were analyzed to evaluate the activity of microbial population of soils. The molecular characterization was carried out by the direct extraction of DNA, gene amplification of the 16S rRNA of the Eubacteria by PCR and specific groups such as the Actinomycetes and Ammonia-oxidizing bacteria. The amplification products were separated by DGGE under appropriate conditions of denaturation and the resulting genetic fingerprinting were analyzed using the software Bionumerics.
Molecular and biochemical characterization of the rhizosphere soil of a closed landfill for its environmental restoration
SPAGNUOLO, Matteo;TERZANO, ROBERTO;CRECCHIO, Carmine;BRUNETTI, Gennaro
2012-01-01
Abstract
A revegetation process of a closed urban solid waste landfill was initiated in 2009 for the environmental restoration of the site. The landfill is located in Foggia district in the North part of the Apulia region. Growth experiments in pots and in open field were carried out on eight plant species in order to select those that could be more appropriate in the particular extreme field conditions (climate, high slope of the walls, bad leachate control). In particular, plant growth was assessed in two different soil types (agricultural and leachate contaminated soil). Among the different species assessed, Pistacia lentiscus L. and Puccinellia borreri. L. were selected because of their performance in the contaminated soil and mostly since they are typical native Mediterranean plant species. Rhizosphere and bulk soil of these two plant species, sampled from the pots, has been characterized by biochemical and molecular methods in order to highlight shift in the composition of the microbial community caused by the presence of leachate. Enzymatic activities (dehydrogenase, fluorescein diacetate hydrolysis, ?-glucosidase and protease) and microbial biomass C were analyzed to evaluate the activity of microbial population of soils. The molecular characterization was carried out by the direct extraction of DNA, gene amplification of the 16S rRNA of the Eubacteria by PCR and specific groups such as the Actinomycetes and Ammonia-oxidizing bacteria. The amplification products were separated by DGGE under appropriate conditions of denaturation and the resulting genetic fingerprinting were analyzed using the software Bionumerics.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
