The present work is aimed at developing gold nanostructures functionalized with antenna systems to exploit the synergistic nanostructure/antenna desorption-ionization efficiency. A potential Matrix- Assisted Laser Desorption Ionisation (MALDI) organic matrix has been modified introducing specific functional groups or molecular linker and used as a capping agent for gold nanostructures. In particular, conjugated naphthyl-thio-derivative, i.e. 4-mercaptonaphthalene-1,8-dicarboxylic acid, was synthesized and characterized by means of nuclear magnetic resonance, UVevisible and X-ray photoelectron spectroscopies. Afterwards, the thio-derivative was used as covalent surface modifier for flat gold surfaces and nanostructured gold films. These surfaces were thoroughly characterized by means of parallel angle-resolved X-ray photoelectron spectroscopy to obtain quantitative information about elemental composition, chemical speciation, and in-depth distribution of the target chemical functional groups. Finally the compound was preliminarily tested as a non-conventional matrix in Laser Desorption Ionisation Mass Spectrometry (LDI-MS) analysis of low molecular weight biomolecules in order to assess its capability of acting as the antenna system and proton donor after covalent bonding to gold nanomaterials.
Designing functionalized gold surfaces and nanostructures for Laser Desorption Ionisation Mass Spectrometry
DITARANTO, NICOLETTA;Monopoli A;NACCI, Angelo;PALMISANO, Francesco;SABBATINI, Luigia;CIOFFI, NICOLA
2014-01-01
Abstract
The present work is aimed at developing gold nanostructures functionalized with antenna systems to exploit the synergistic nanostructure/antenna desorption-ionization efficiency. A potential Matrix- Assisted Laser Desorption Ionisation (MALDI) organic matrix has been modified introducing specific functional groups or molecular linker and used as a capping agent for gold nanostructures. In particular, conjugated naphthyl-thio-derivative, i.e. 4-mercaptonaphthalene-1,8-dicarboxylic acid, was synthesized and characterized by means of nuclear magnetic resonance, UVevisible and X-ray photoelectron spectroscopies. Afterwards, the thio-derivative was used as covalent surface modifier for flat gold surfaces and nanostructured gold films. These surfaces were thoroughly characterized by means of parallel angle-resolved X-ray photoelectron spectroscopy to obtain quantitative information about elemental composition, chemical speciation, and in-depth distribution of the target chemical functional groups. Finally the compound was preliminarily tested as a non-conventional matrix in Laser Desorption Ionisation Mass Spectrometry (LDI-MS) analysis of low molecular weight biomolecules in order to assess its capability of acting as the antenna system and proton donor after covalent bonding to gold nanomaterials.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
